Journal: Frontiers in Aging

Article Title: The truncated isoform of the receptor for hyaluronan-mediated motility (RHAMM Δ163 ) modulates shelterin and telomerase reverse transcriptase transcription affecting telomerase activity

doi: 10.3389/fragi.2025.1604051

Figure Lengend Snippet: RHAMM Δ163 regulates telomerase-mediated telomere elongation. RHAMM is present in multiple isoforms, including RHAMM FL and RHAMM Δ163 in mice (A) . HMMR was eliminated using CRISPR-Cas9 in MDA-MB-231 cells. RNA was isolated, and qRT-PCR was used to measure the mRNA expression of TERT, TPP1 , and POT1 , normalized to Gapdh . Data are means ± SD; n = 3 experiments; *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test (B,F,I) . RNA was also isolated from 10T1/2, LR21, RHAMM Δexon8-16 , KOV4, and KOV5 cell lines, with mRNA levels for mTert , Pinx1, Sirt1, Tpp1 , and Pot1a measured similarly (C,D,E,G,J) . For RHAMM Δexon8-16 (KO) and rescued lines (KOV4 and KOV5), treatment with PD98059 at 50 µM for 24 h was followed by qRT-PCR analysis to measure the mRNA expression of Tpp1 , and Pot1a , normalized to Gapdh . Results are means ± SD; n = 2 experiments; *p < 0.05, **p < 0.01, ***p < 0.001 (H,K) .

Article Snippet: The TRAPeze Gel-based Telomerase Detection Kit (Cat #S7700, Millipore Sigma, United States) was used to perform the telomerase repeat amplification protocol (TRAP) assay according to the manufacturer’s instructions.

Techniques: CRISPR, Isolation, Quantitative RT-PCR, Expressing

Journal: Frontiers in Aging

Article Title: The truncated isoform of the receptor for hyaluronan-mediated motility (RHAMM Δ163 ) modulates shelterin and telomerase reverse transcriptase transcription affecting telomerase activity

doi: 10.3389/fragi.2025.1604051

Figure Lengend Snippet: Telomerase activity affects RHAMM expression. Cycloastragenol (3 µM) was added to 10T1/2 fibroblasts for 6 h, followed by RNA isolation and qRT-PCR analysis to measure the mRNA expression of Hmmr, mTert, Tpx2 , Aurka , and Has2, normalized to Gapdh or Snrpd3 . Data are means ± SD; n = 3 experiments; *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test (A–E) . The extracellular hyaluronan concentration was analyzed by hyaluronan-based ELISA assay. The hyaluronan level was normalized by total RNA concentration (F) .

Article Snippet: The TRAPeze Gel-based Telomerase Detection Kit (Cat #S7700, Millipore Sigma, United States) was used to perform the telomerase repeat amplification protocol (TRAP) assay according to the manufacturer’s instructions.

Techniques: Activity Assay, Expressing, Isolation, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay